• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The raw grassy and bitter principles in soybeans are reduced to acceptable levels for purposes of human consumption without significant degradation of the nutritional properties. This result is achieved by a lipid extraction process in which raw soybean material is treated with carbon dioxide under carefully controlled supercritical conditions. Of particular importance are the moisture content of the bean material as well as the pressure, temperature, and contact time of the carbon dioxide extractant.
    公开号:SU1489570A3
    申请号:SU853894715
    申请日:1985-05-17
    公开日:1989-06-23
    发明作者:Dzhon Fillip Fridrikh；Artur Charlz Eldridzh
    申请人:Dzhon Fillip Fridrikh；Artur Charlz Eldridzh；
    IPC主号:
    专利说明:

    The invention relates to the oil industry and relates to the production of fat-free soy product. The goal is to improve the quality of the protein product. This is achieved in that the soybeans are crushed, extracted with carbon dioxide under a pressure of 740.25-848.82 atm under supercritical conditions, the mixture is heated to 81-100 ° C, and the soybeans are used for extraction with a moisture content of 9-12% .. 2 tabl>
    The invention relates to the oil and fat industry and relates to the production of low-fat products.
    The purpose of the invention is to improve the quality of the protein product.
    The method is as follows
    Soybean seeds are cleaned, crushed and sent to the extractor, the extraction is carried out at a pressure of 740.25 - · -,
    848.82 atm and heating the mixture to 81100 ° C, soybeans for extraction are used with a moisture content of 912%, then the extract is divided into a fat fraction and a protein product.
    One of the fundamental problems in the development of soybean fractionation as the final stage of obtaining plant protein products for human use is the inherent flavor of soy products. Traditional solvent extraction methods
    2
    using hexane or other hydrocarbons, the components of the raw materials and components are left in the flour ,. responsible for the bitter taste. In addition, Lippes in flour tend to auto-or enzymatic oxidation to compounds that contribute to the development of unfavorable taste. The result is a final reduction in the organoleptic and nutritional qualities of the food.
    Lipoxygenase is an enzyme, partly responsible for the deterioration of oxidation. Lipoxygenase can be inactivated by heat, but this spo: GSS is not satisfactory due to the fact that heat (drying) reduces the protein solubility as determined by the nitrogen solubility index (N51) of the initial value
    5. "1489570 A 5
    3
    1489570
    four
    for raw beans 85-90 to unacceptable levels below 60.
    Explore alternative solvents such as ethanol and isoprop-a. but they cannot compete with hexane, either in terms of solvent properties or in terms of extraction. This invention relates to a method of working out soybeans, such as extracting oil, and simultaneously obtaining high-quality nutritional protein components about
    In soybean lipid extraction of carbon dioxide with carefully controlled supercritical conditions, the proportion of raw materials and components that give a bitter taste typical of skimmed flour are significantly reduced without an undesirable decrease in protein solubility, as measured by N81. As a result, the flour, as well as the concentrates and the selected components obtained from the flour, have acceptable palatability.
    Accordingly, the invention allows for the provision of high quality nutritional protein soy products having high N81 values and organoleptic acceptability for human consumption.
    Soybeans that are extracted must first be converted to a physical state that is permeable to carbon dioxide under supercritical conditions. Whole beans are processed by grinding, peeling and peeling. The initial moisture content in the resulting material is at least 6.5 mas. For all practical purposes, the moisture content may be in the range of 6.5-15 wt.%. The optimum moisture content range is 9-12 masd.
    For the implementation of the proposed method is suitable for any standard SSR equipment, including an extractor and a separator. In a typical mass production, material from soybeans is loaded into an extractor, which is hermetically sealed, and then CS-CO ^ is introduced under predetermined conditions.
    Temperature, pressure, moisture content, flow rate. The characteristics are integrally relative and
    should be chosen so as to control the preferential release of oil from the bean material with a minimal decrease in protein solubility and the achievement of acceptable palatability.
    The invention allows to obtain a fat-free product, characterized by a high content of soluble nitrogen and a reduced amount of raw materials and components with a bitter taste compared with the original legume material. In defatted products, a residual oil level of D or less is acceptable, and N81 values are greater than or equal to 60, Tastes are usually rated on a 10-point scale - with 10 as “soft” (excellent quality) and 1 as “strong” (poor quality). The overall taste rating should be equal to or greater than 6.0. Estimation of taste of individual intensity (Р1У) is considered on a three-point scale (from 0 to 3), and 0 corresponds to the absence of taste and 3 - to a strong intensity of taste. Untreated raw materials and a bitter taste Ρίν of 1.5 or less are in an acceptable area.
    Processing time is an inverse function of the flow 8C-CO g . The flow rate is chosen in such a way as to minimize the residence time of the bean material in the extractor at elevated temperatures without significant loss of product quality. Under some conditions, time may be a large amount in order to reduce the proportion of raw materials and components with a bitter aftertaste to the desired level compared to the level focused on obtaining oil. Preselection; working parameters of pressure, temperature and moisture content, determining the balance of flow rate and processing time carried out by known methods. Flow rates of 40-100 standard liters per 1 minute per 1 kg of material (40,100 tbsp / min / kg of material) are adequate,
    The extreme upper limit of pressure is limited only by equipment and economy. However, the most suitable pressure is a pressure in the range of 750-860 bar 740.25848.82 atm. Working temperature
    1489570
    the interval is 81-100 ° C. Below 81 υ 0, overall taste is sharply falling and, above 100 ° C, the ability of the protein to denature results in low N81 values. Optimum temperatures are in the range of 81-84 ° C. Although the preferred pressure range is 755-860 bar (745.185-848.82 atm), the content of
    moisture and temperature should be varied inversely with each other, i.e. moisture and temperature cannot represent together the same extreme limits in their range. When both of these quantities are in the lower limit, the taste of the product is bad, when both of these values are in the upper limit, the value of N81 is unusually reduced. 20
    During ZS-C0 2 treatment, lipids are first extracted, and then water.
    In mass production, the material at the end of the liquid inlet to the extractor is first released from the oil. The front 25 of the highest extraction rate then progresses through the material to the exit of the extractor. As a result, the inlet moisture content sometimes falls below an acceptable level to remove raw materials and components with a bitter aftertaste. In this case, it is desirable to moisten the feed solvent. For systems equipped with an inlet filter, an effective humidification method is to saturate the filter with water before it enters the process. Other methods of moistening the input C0 2 are also known.
    The solubility of the oil in C0 2 is a function of temperature and pressure and, under fixed conditions, it remains virtually constant during most of the extraction. However, when the residual oil in the material 45 of soybeans approaches zero, an apparent solubility occurs,
    Containing oil CS-C0 2 stream flows from the extractor to the separator for subsequent extraction. Fat-free residue - soy flakes extracted from the extractor is dry and very brittle and easily crushed into fine, highly dispersed flour, which is used in beverages, in bakery products, in pastes in whipped dough. Flour contains 4950 ma. I protein, characterized by a value of N81 at least 60 and
    lipoxygenase activity close to zero.
    Fat-free flakes are suitable for conversion to high-quality protein concentrates using any of several methods known in the art. In the process of obtaining a concentrate, proteins are immobilized and sugar and other soluble components are removed.
    In addition, a defatted soy residue can be used to extract high quality protein.
    known methods.
    Examples 1-15. Pure conditioned soybean seeds are crumbled, sheared and turned into flakes up to 0.25 mm, passing through a smooth ring mill. The flakes are then adjusted to the desired level of moisture by adding or removing the appropriate amount of water. For each experiment, 35 g of these flakes are placed in an extraction tube with a length of 560 mm and an inner diameter of 14.4 mm (nominal pressure 1380 bar, 1362.06 mm).
    A glass fiber cork filter is placed at each end of the tube. To the cork filter add 1 ml of water on the inlet side of the extractor (top of the extractor). Dry industrial C0 2 at the target pressure is passed through flakes with a speed of 6 tbsp / min (171 tbsp / min / κι) for 20 minutes, maintaining the target temperature with an accuracy of 11-2 C. The flow rate and processing time are preselected in order to ensure complete extraction in all experiments. Extracted oil is extracted from C0 2 in a separator located at 60 ° C. Oil yields were in the range of 22–56–22.91% of the dry basis weight of the original flakes for all 14 experiments with residual oil in the extracted flour less than 1Ζ. The defatted soy flakes are removed from the extractor and crushed to 100 mesh for subsequent evaluation and analysis.
    N51 is determined according to ACC5 method Ba11-65.
    Samples of the tested soy protein products for taste are evaluated according to a known method (Magpeg e! A1, Segea1. Shesh. 1983, 60 (2), D02-106). Products are tested as a 2% dispersion in filtered water through activated carbon at rooms7
    1489570 8
    Noah temperature. Individual taste qualities of samples are evaluated on a scale of 0-3 (0 - no taste, 1 - weak, 2 - medium, 3 - strong taste in intensity). The magnitude of the intensity of taste (Ρίν), which is an average weight, calculated according to the following formula:
    1x (number of weak responses) + y · +2 x (number of average responses) +3 x (number of strong from ΡΙν = _Κϋί "° Βΐ -------------------- The number of test samples
    15
    The overall score is based on a 10-point scale: 10 - as soft (excellent quality) and 1 - as strong (poor quality). Evaluation of raw materials and the quality of raw materials are equalized in order to take into account the changes among the tested subjects of 20 raetsov. Balanced calculations are used as an evaluation test for 14 experiments. When testing is evaluated three samples from each batch: each ob- 25 sample was evaluated nine times to calculate the total average rating.
    The test results are presented in Table 1 and 2 "
    Example 15. A sample of conditioned soybean seeds is crumbled, husked and solids are passed through a smooth ring mill to produce flakes with a thickness of 0.025-0.04 mm, which contain 19.9% oil, 41.2% protein and 11.2% moisture. 1500 g of sample 35 is placed in a 4-liter extractor, which is then hermetically sealed and fed there a pressure of 862 bar (850,
    794 atm), at the same time heating it with 4 θ at a controlled temperature of 82 ° C. The pressure in the C0 2 cylinder 7683 bar (75,012-81,921 atm) and a flow rate of 67 st, l / min / kg was maintained for 20 min. The separator (reci- - 45 ver) is heated at 60 ° C to remove the extracted water from the recoverable oil. The amount of consumption of C0 2 and extraction of oil is measured periodically at intervals, allowing 50
    They are able to calculate the oil extraction rate and oil solubility.
    These values remain constant until the residual oil is a few percent of the 55 level. The defatted flour is removed from the extractor and milled to obtain a 100 mesh powder. Extracted powder contains 0.82%
    residual oil and 8.1% moisture. The powder is further characterized using N81 of 63.1 and a total flavor rating of 7.3 when evaluating the results of a series of experiments.
    Example 16 In order to evaluate the functional and sensory properties of 8C-C0g extracted soybean powder, 400 g of the powder obtained in Example 15 is mechanically stirred in 4 liters of water at room temperature, and the suspension is gradually adjusted to pH 7.4-7.6 with alkali for 1 h stirring. The suspension is then centrifuged, and the separated solids are stirred in an additional 2000 ml of water for 30 minutes before centrifuging. The two upper centrifugates are combined and hydrochloric acid is added to lower the pH to 4.2-4.7. The precipitated protein is separated by centrifugation and the wet, cheesy precipitate is separated. The curd is dispersed in 400 ml of distilled water and the pH of the protein suspension is adjusted to 7.1 with alkalis. The proteinate is subjected to drying in the frozen state to obtain sodium protein.total product of 105.2 g, which is a yield of 26.3% based on the original 8C-C0 ^ extracted flakes. The resulting individual component is evaluated in examples 1-15. The overall flavor estimate is 6.1 with no share of raw materials.
    Example 17. Soy protein concentrate is obtained by suspending 100 g of soy flakes extracted in Example 15 in 3 l of water. The pH was adjusted to 4.5 with stirring of the sample for 1.5 hours. The insoluble portion was separated by centrifugation, then resuspended in water and then dried in a frozen state. The product is characterized by an overall taste rating of 6.1 with a serial rating.
    Example 18. A second soy protein concentrate is obtained by suspending 100 g of soy flakes extracted in Example 15 in 3 liters of 60% aqueous ethanol. After stirring for 1.5 hours, the suspension is centrifuged and the residue is dispersed in water and then dried in a frozen state. The product is characterized by a total
    '1489570
    9
    estimated taste of 7.2 in the serial assessment of
    Example 19, The third soy protein concentrate is obtained by heating 100 g of soybean flakes extracted in Example 16 in an autoclave at 121 ° C and a pressure of 1 bar (0.987 atm) for 15 minutes. After heating, the sample is washed with 3 liters of water and centrifuged. The recovered solids are suspended in water and the concentrate is separated by drying in a frozen state. The product has an overall flavor rating of 6.8 15
    during serial testing.
    ten
    F.
    权利要求:
    Claims (1)
    [1]
    formula of the invention. Method for producing fat-free
    soy protein product, including grinding of soybeans, extraction with carbon dioxide under supercritical conditions under pressure when heated and separation of the extract into a fat fraction and the target product, characterized by
    the fact that, in order to improve the quality of the protein product, during extraction, the pressure is maintained at 740.25-848.82 atm, and the mixture is heated to 81-100 ° C, and soybeans are used with a moisture content of 9-12%.
    Table 1
    When- Pressure Temperature Content N81 * Lead measures air, atm ra, cmoisture% dayassessmenttaste
    one 742.84 84 2 742.84 84 ' 3 742.8496 > four 742.84 96 * five 777.14 80 I ** *** 6 777.14 90 7 777.14 90 eight 777.14 90 9 777.14 100** ten 810.46 84 ** eleven 810.46 84 12 810.46 96 13 810.46 96 ** 14 837.90 90
    6.5 ** 79.7 4.2
    11.4 63.3 6.1
    6.5 66,8 6.8 C 4 32.5 7.2 9.0 80.2 5.8 5.0 ** "' 82.0 4.8 9.0 67.5 6,6 12.4 61.6 6.4 9.0 ** 48.0 6.9 6.5 ** 80.7 5.6 11.4 62.4 7.2 6.5 72.0 6.0 11.4 34.3 7.3 9.0 69.4 6,6
    * N81 (nitrogen solubility index) £ 60. The given flavor estimate is: "6.0. Ρίν (intensity of taste) έ 1.5.
    ** Parameter combination not acceptable.
    *** Individual parameters are unacceptable.
    ч 1489570 ’2
    table 2
    Example πν * Lipoxygenase activity, 2 from initial 4 Fields of raw materials, 2 Bittersmack one 2.4 0.9 20 2 1.2 0.9 0 3 0.9 0.7 0 four 0.6 0.6 0 five· 1.0 0.9 17 6 2.2 1,3 9 7 0.9 0.8 0 eight 0.8 0.9 0 9 0.6 0.2 0 ten 1.4 0.8 14 eleven 0.4 0.6 0 12 1.0 1.1 0 13 oh w 0.4 0 14 0.8 m 0
    N81 (nitrogen solubility index) £ 60. The given flavor estimate)> / 6.0, Ρίν (flavor intensity value) <1.5.
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    US06/534,015|US4493854A|1983-09-20|1983-09-20|Production of defatted soybean products by supercritical fluid extraction|
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